VitaLyse® is a reagent system designed to lyse erythrocytes in peripheral blood, lymph nodes, bone marrow, umbilical cord blood and other biological specimens. Leukocytes recovered after lysing treatment are fully viable and ready for flow cytometric immunophenotypic analysis, fluorescence-activated cell sorting or tissue culture. VitaLyse maintains complete viability and biological function of leukocytes without activation. VitaLyse has been shown to be especially useful in removing erythrocytes from bone marrow samples in the preparation of fragile and immature cells for long-term colony assays and in the preparation of T-cells for long-term activation cultures. If desired, cells for flow cytometric analysis can also be fixed (included in kit) and stored at 2-8° C for later analysis. VitaLyse is ideal for use in conjunction with PrepaCyte separated samples. For research use only.
Proof of Performance
Light scatter of a normal ACD blood sample processed with VitaLyse
CD45 versus CD14 showing 98% purity in the lymphocyte gate
CD45 gating of a normal ACD blood sample processed with VitaLyse
CD45 versus CD14 staining of leukocytes